製品の概要

  • 製品名Anti-CREB3L1 antibody
    CREB3L1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CREB3L1
  • 特異性ab33051 recognises CREB3L1.
  • アプリケーション適用あり: ELISA, WB, IHC (PFA fixed), IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: FTENMEDFSN DLFSSFFDDP VLDEKSPLLD MELDSPTPGI QAEHSYSLSG, corresponding to N terminal amino acids 43-92 of Human CREB3L1

  • ポジティブ・コントロール
    • Fetal small intestine lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC (PFA fixed) Use at an assay dependent concentration. PubMed: 25359725
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE.
  • 組織特異性Ubiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain.
  • 配列類似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • 翻訳後修飾Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus.
  • 細胞内局在Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BBF-2 homolog antibody
    • CAMP responsive element binding protein 3 like 1 antibody
    • cAMP-responsive element-binding protein 3-like protein 1 antibody
    • CR3L1_HUMAN antibody
    • Creb3l1 antibody
    • Cyclic AMP responsive element binding protein 3 like protein 1 antibody
    • OASIS antibody
    • Old astrocyte specifically-induced substance antibody
    • Processed cyclic AMP-responsive element-binding protein 3-like protein 1 antibody
    see all

Anti-CREB3L1 antibody 画像

  • Anti-CREB3L1 antibody (ab33051) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab33051 staining CREB3L1 in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked using 1.5% serum for 1 hour at 25°C and then incubated with ab33051 at a 1/800 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated polyclonal.

    See Abreview

  • Immunofluorescence analysis of U2-OS, staining CREB3L1 with ab33051.

    Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 in PBS and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA in PBS) for 12 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

Anti-CREB3L1 antibody (ab33051) 使用論文

This product has been referenced in:
  • Delgado-Olguín P  et al. Ezh2-mediated repression of a transcriptional pathway upstream of Mmp9 maintains integrity of the developing vasculature. Development 141:4610-7 (2014). IHC (PFA fixed) ; Mouse . Read more (PubMed: 25359725) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - 0.5% NP40/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (primary vascular smooth muscle cells)
Specification primary vascular smooth muscle cells
Fixative Methanol/Acetone
Permeabilization Yes - 0.5% NP-40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

投稿 Mar 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Vector Labs Unmasking Solution H-3300
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

投稿 Mar 19 2012

Thank you for your query and I am very sorry for the delay in getting back to you.


The antibody which your customer is interested in, anti-CREB3L1 antibody (ab33051), was raised against a peptide taken from the amino acid residues 43-92...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SAOS-2, U2OS, HEK 293T, ATDC5)
Loading amount 15 µg
Specification SAOS-2, U2OS, HEK 293T, ATDC5
Treatment 0.02 ug/ml tunicamycin for 72h
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

投稿 Nov 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"