製品の概要

  • 製品名Anti-CREB3 antibody
    CREB3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CREB3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CREB3.

    (Peptide available as ab91077.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. An additional transcript variant has been identified, but its biological validity has not been determined.
  • 細胞内局在Isoform 2: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Membrane. Isoform 3: Nucleus. Cytoplasm.
  • 参照データベース
  • 別名
    • Basic leucine zipper protein antibody
    • cAMP responsive element binding protein 3 antibody
    • Clic AMP responsive element binding protein 3 antibody
    • CREB 3 antibody
    • cyclic AMP response element (CRE) binding protein/activating transcription factor 1 antibody
    • Cyclic AMP responsive element binding protein 3 antibody
    • Leucine zipper protein antibody
    • LUMAN antibody
    • LZIP antibody
    • MGC15333 antibody
    • MGC19782 antibody
    • Transcription factor LZIP alpha antibody
    see all

Anti-CREB3 antibody 画像

  • All lanes : Anti-CREB3 antibody (ab78182) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab78182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78182, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.

Anti-CREB3 antibody (ab78182) 使用論文

ab78182 has not yet been referenced specifically in any publications.

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Expiration date: 19-07-2012
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"