Anti-CREB (phospho S129 + S133) 抗体 (ab10564)
Key features and details
- Rabbit polyclonal to CREB (phospho S129 + S133)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CREB (phospho S129 + S133) antibody
CREB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CREB (phospho S129 + S133) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CREB (phospho S129 + S133).
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ポジティブ・コントロール
- WB: NIH3T3 cell extract with various treatments; A549 cell extract with various treatments; NIH3T3, A549, SK-N-SH, MCF7, and MDA-MB-231 untreated, EGF-treated or PDGF-treated cell lysates. ICC/IF: 70% confluent log phase HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
PBS is Ca2+ and Mg2+ free -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a spanning peptide corresponding to the cleavage-site to remove antibody that is reactive with non-phosphorylated CREB. The final product is generated by affinity chromatography using a CREB-derived peptide that is phosphorylated at serines 129 and 133. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/500.
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WB | (2) |
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).
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特記事項 |
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ICC/IF
1/100 - 1/500. |
WB
1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. -
関連疾患
Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. -
配列類似性
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain. -
翻訳後修飾
Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1385 Human
- Entrez Gene: 12912 Mouse
- Omim: 123810 Human
- SwissProt: P16220 Human
- SwissProt: Q01147 Mouse
- Unigene: 516646 Human
- Unigene: 453295 Mouse
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別名
- Active transcription factor CREB antibody
- cAMP response element binding protein 1 antibody
- cAMP response element binding protein antibody
see all
画像
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All lanes : Anti-CREB (phospho S129 + S133) antibody (ab10564) at 1/1000 dilution
Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract
Lane 3 : NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4 : NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, incubated with a generic phosphoserine-containing peptide
Lane 5 : NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, incubated with the phosphopeptide immunogen
Lane 6 : NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, treated with lambda phosphatase
Predicted band size: 37 kDaThe membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method.
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All lanes : Anti-CREB (phospho S129 + S133) antibody (ab10564) at 0.5 µg/ml
Lane 1 : Human A549 whole cell extract
Lane 2 : Human A549 whole cell extract stimulated with EGF
Lane 3 : Human A549 whole cell extract treated with pervanadate
Lysates/proteins at 20000 cells per lane.
Secondary
All lanes : Anti-mouse, HRP-conjugated
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds -
All lanes : Anti-CREB (phospho S129 + S133) antibody (ab10564) at 1/1000 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NIH/3T3 treated for 10 minutes with 50 ng/mL of PDGF cell lysate
Lane 3 : NIH/3T3 treated for 10 minutes with 200 ng/mL of EGF cell lysate
Lane 4 : A549 cell lysate
Lane 5 : A549 treated for 10 minutes with 200 ng/mL of EGF cell lysate
Lane 6 : A431 cell lysate
Lane 7 : SK-N-SH cell lysate
Lane 8 : MCF7 cell lysate
Lane 9 : MDA-MB-231 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG - HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 35,43 kDa why is the actual band size different from the predicted?Detection: Chemiluminescence.
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Immunofluorescent analysis of CREB (phospho S129/pS133) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with CREB (phospho S129/pS133) Rabbit polyclonal Antibody (ab10564) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab10564 は 7 報の論文で使用されています。
- Zhao J et al. Repeated exposure to sevoflurane in neonatal rats impairs cognition in adulthood via the PKA-CREB-BDNF signaling pathway. Exp Ther Med 22:1442 (2021). PubMed: 34721684
- Peres RS et al. TGF-ß signalling defect is linked to low CD39 expression on regulatory T cells and methotrexate resistance in rheumatoid arthritis. J Autoimmun 90:49-58 (2018). WB . PubMed: 29426578
- Zimmermann HR et al. Genetic removal of eIF2a kinase PERK in mice enables hippocampal L-LTP independent of mTORC1 activity. J Neurochem 146:133-144 (2018). PubMed: 29337352
- Sun Z et al. Norepinephrine inhibits the cytotoxicity of NK92-MI cells via the ß2-adrenoceptor/cAMP/PKA/p-CREB signaling pathway. Mol Med Rep 17:8530-8535 (2018). PubMed: 29658580
- Hostrup M et al. Beta2 -adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men. J Physiol 596:4121-4139 (2018). PubMed: 29968301
- Su Y et al. Receptor desensitization and blockade of the suppressive effects of prostaglandin E(2) and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes. Cancer Immunol Immunother 60:111-22 (2011). WB ; Human . PubMed: 20960188
- Harmon EB et al. Beta-adrenergic receptor activation in immortalized human urothelial cells stimulates inflammatory responses by PKA-independent mechanisms. Cell Commun Signal 3:10 (2005). WB . PubMed: 16091148