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Our Abpromise guarantee covers the use of ab10564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).|
Immunohistochemistry (PFA perfusion fixed frozen section) staining of CREB (phospho S129 + S133) in the mouse cortex. Samples were fixed with paraformaldehyde and ab10564 was used at 1/1000 for 18 hours at 25ºC. An Alexa Fluor®568 conjugated donkey anti-Rabbit secondary was used at 1/1000.