製品の概要

  • 製品名Anti-CREB antibody
    CREB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CREB
  • 特異性This antibody detects endogenous levels of total CREB protein.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized non phosphopeptide derived from human CREB around the phosphorylation site of serine 129 (I-L-SP-R-R).

  • ポジティブ・コントロール
    • IHC-P: Human breast carcinoma tissue WB: NIH/3T3 cell extract

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

ターゲット情報

  • 機能This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • 関連疾患Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • 配列類似性Belongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻訳後修飾Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all

Anti-CREB antibody 画像

  • This image shows human breast carcinoma stained with ab47781 at 1/50 dilution. Right hand image: tissue was treated with immunogenic peptide. Left hand image: untreated.
  • All lanes : Anti-CREB antibody (ab47781) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract, untreated.
    Lane 2 : NIH/3T3 cell extract, treated with immunogenic peptide.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa

Anti-CREB antibody (ab47781) 使用論文

ab47781 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab47781.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"