製品の概要

  • 製品名Anti-CREB antibody [48H2]
    CREB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [48H2] to CREB
  • アプリケーション適用あり: WB, IP, IHC-Fr, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    CREB full length fusion protein.

  • ポジティブ・コントロール
    • SK-N-MC, NIH-3T3, and COS cell extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 37 kDa.
IP 1/250.
IHC-Fr 1/400.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/1600.
Flow Cyt 1/400. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • 関連疾患Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • 配列類似性Belongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻訳後修飾Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all

Anti-CREB antibody [48H2] 画像



  • Predicted band size : 37 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CREB knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab49762 observed at 44 kDa. Red - loading control, ab18058, observed at 124 kDa.
    ab49762 was shown to recognize CREB when CREB knockout samples were used, along with additional cross-reactive bands. Wild-type and CREB knockout samples were subjected to SDS-PAGE. ab49762 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • ab49762 staining CREB in rat brain cortex and cerebellum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed using a zinc fixative and blocked with biotin for 16 minutes at 37°C. Samples were incubated with primary antibody (9 ug/mL) for 1 hour at 37°C. A biotinylated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

Anti-CREB antibody [48H2] (ab49762) 使用論文

ab49762 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain Cortex and cerebellum)
Permeabilization No
Specification Brain Cortex and cerebellum
Blocking step Ventana Endogenous Biotin Blocking Kit (cat.# 760-050) as blocking agent for 16 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative BD Pharmigen IHC Zinc Fixative (cat.# 51-7538KZ)
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投稿 Feb 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"