製品の概要

  • 製品名Anti-Creatine kinase MT antibody
    Creatine kinase MT 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Creatine kinase MT
  • アプリケーション適用あり: WB, ELISA, IHC-Fr, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Chicken, Human
    交差が予測される動物種: Mouse, Rat, Horse, Dog, Rhesus monkey
  • 免疫原

    Recombinant fragment: GVHIKLPLLS KDSRFPKILE NLRLQKRGTG GVDTAATGGV FDISNLDRLG KSEVELVQLV IDGVNYLIDC ERRLERGQDI RIPTPVIHTK H, corresponding to amino acids 327-418 of Human Creatine kinase MT (NP_066270) with a tag of 26 kDa.

  • ポジティブ・コントロール
    • A-431 cell lysate. Creatine kinase MT transfected 293T cell lysate. Recombinant tagged Human Creatine kinase MT fragment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 46 kDa. A 72kDa faint band could be seen in WB. We are unsure of its identity.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Creatine kinase MT is approximately 0.1ng/ml if used as a capture antibody.
IHC-Fr 1/2500.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.
  • 細胞内局在Cell Membrane and Mitochondrial
  • 参照データベース
  • 別名
    • Acidic type mitochondrial creatine kinase antibody
    • CKMT antibody
    • CKMT1 antibody
    • CKMT1A antibody
    • CKMT1B antibody
    • Creatine kinase mitochondrial 1 ubiquitous antibody
    • Creatine kinase U type, mitochondrial antibody
    • Creatine kinase ubiquitous mitochondrial antibody
    • Creatine kinase, mitochondrial 1 (ubiquitous) antibody
    • Creatine kinase, mitochondrial 1A antibody
    • Creatine kinase, mitochondrial 1B antibody
    • Mia CK antibody
    • U MtCK antibody
    • Ubiquitous mitochondrial creatine kinase antibody
    • UMTCK antibody
    see all

Anti-Creatine kinase MT antibody 画像

  • Anti-Creatine kinase MT antibody (ab76506) at 1 µg/ml + Recombinant tagged human Creatine kinase MT fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 46 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-Creatine kinase MT antibody (ab76506) at 1 µg/ml + A-431 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Creatine kinase MT antibody (ab76506) at 1 µg/ml

    Lane 1 : Creatine kinase MT transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Creatine kinase MT antibody (ab76506) at 1 µg/ml

    Lane 1 : Creatine kinase MT over-expressed 293 cell line, non-transfected control
    Lane 2 : Creatine kinase MT over-expressed 293 cell line, cotransfected with Creatine kinase MT Validated Chimera RNAi

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab76506 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab76506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76506, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Creatine kinase MT antibody (ab76506) 使用論文

This product has been referenced in:
  • Branco AF  et al. Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype. PLoS One 10:e0129303 (2015). Read more (PubMed: 26121149) »
  • Bravo C  et al. Metabolomic analysis of two different models of delayed preconditioning. J Mol Cell Cardiol 55:19-26 (2013). WB . Read more (PubMed: 23127662) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - other (Brain and liver homogenates vs isolated mitochondr)
Specification Brain and liver homogenates vs isolated mitochondr
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Marco Spinazzi

Verified customer

投稿 Mar 25 2015

Application Western blot
Sample Rat Cell lysate - whole cell (H9c2 cell line)
Loading amount 25 µg
Specification H9c2 cell line
Gel Running Conditions Reduced Denaturing (14% separating gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Apr 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 60 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 14 2012

DISCOUNT CODE: *** Expiration date: March 30th, 2012 I am very pleased to hear you would like to accept our offer and test ab76506 in chicken. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please subm...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"