製品の概要

  • 製品名
    Anti-Creatine Kinase MM antibody
    Creatine Kinase MM 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Creatine Kinase MM
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: TCPSNLGTGL RGGVHVKLAH LSKHPKFEEI LTRLRLQKRG TGGVDTAAVG SVFDVSNADR LGSSEVEQVQ LVVDGVKLMV EMEKKLEKGQ SIDDMIPAQK , corresponding to amino acids 282-382 of Human Creatine Kinase MM

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab189438 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 配列類似性
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CKM antibody
    • CKMM antibody
    • Creatine kinase M antibody
    • Creatine kinase M chain antibody
    • Creatine kinase M type antibody
    • Creatine kinase M-type antibody
    • Creatine kinase muscle antibody
    • Creatine kinase, muscle type antibody
    • KCRM_HUMAN antibody
    • M-CK antibody
    • MCK antibody
    • Muscle creatine kinase antibody
    see all

画像



  • Predicted band size : 43 kDa
    Creatine Kinase MM antibody (ab54637) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54637 at 10 µg/ml staining Creatine Kinase MM in human Hela cells by Immunocytochemistry / Immunofluorescence.

参考文献

This product has been referenced in:
  • Zhang GJ  et al. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood. Biosens Bioelectron 28:459-63 (2011). Read more (PubMed: 21807497) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

投稿 Oct 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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