製品の概要

  • 製品名
    Anti-Creatine Kinase MB antibody
    Creatine Kinase MB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Creatine Kinase MB
  • 由来種
    Rabbit
  • 特異性
    ab31832 will detect CK-MB,CK-MM and CK-BB
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution (PMID 18611857).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Creatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.
    • 細胞内局在
      Cytoplasmic and Mitochondrial
    • 参照データベース
    • 別名
      • B-CK antibody
      • brain creatine kinase antibody
      • CKBB antibody
      • CKMB antibody
      • CKMM antibody
      • creatine kinase B antibody
      • creatine kinase brain antibody
      • creatine kinase M antibody
      • Creatine kinase MB antibody
      • creatine kinase muscle antibody
      • M-CK antibody
      • muscle creatine kinase antibody
      see all

    画像

    • The glutathionylated ~ 45 kDa band is sarcomeric mitochondrial creatine kinase, not a complex I subunit. Mitochondria (20 mg protein/ml) were first incubated with [35S]GSH (40 µCi/ml) in STE for 30 minutes on ice, pelleted, and washed in 250 mM sucrose, 1 mM EGTA, 10 mM Tris-HCl, pH 7.4. The bovine and rat mitochondria were incubated ± 0.5 mM diamide for 5 minutes at 37ºC and then with 50 mM NEM for a further 5 minutes. Mitochondria were pelleted and complex I was isolated by BN-PAGE and non-reducing SDS-PAGE and electrotransferred onto nitrocellulose. [35GSH]- containing protein bands were detected using a phosphor imager. The membrane was blocked in PBST (PBS, 0.05% (v/v) Tween 20 with 1% (w/v) skimmed milk powder) and incubated with ab31832 at 1.25 µg/ml, for 1–2 hours at room temperature. Blots were incubated with 1/5000–1/10,000 dilutions of the appropriate secondary anti-serum for 1 hour at room temperature, treated with the chemiluminescent reagent (ECL or ECL Plus) and vi

    プロトコール

    参考文献

    This product has been referenced in:
    • Hurd TR  et al. Complex I within Oxidatively Stressed Bovine Heart Mitochondria Is Glutathionylated on Cys-531 and Cys-704 of the 75-kDa Subunit: POTENTIAL ROLE OF CYS RESIDUES IN DECREASING OXIDATIVE DAMAGE. J Biol Chem 283:24801-15 (2008). WB ; Human . Read more (PubMed: 18611857) »

    See 1 Publication for this product

    レビューと Q&A

    Application
    ELISA
    Sample
    Human Purified protein (Purified native Human CKMB protein)
    Specification
    Purified native Human CKMB protein
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Capture)
    Username

    Mr. Chang Su Jeon

    Verified customer

    投稿 Aug 20 2015

    Thank you for contacting us.

    The protein ab73649 is Creatine Kinase MB Isoenzyme Type-I and protein ab73650 is Creatine Kinase MB, type 2. Theoratically both will work; customer can buy any of these.

    I hope this information is helpful...

    Read More

    Thank you for your email.

    ab31832 and ab19603 has not been tested together in sandwich ELISA so I am sorry we will not be able to confirm it.

    We haven't tested ab73649 and ab72650 as standards with antibodies ab31832 and ab19603. Yo...

    Read More

    Thank you for your enquiry and your interest in our products.

    I can confirm that ab31832 has been tested in ELISA (indirect ELISA) and but not in sandwich ELISA system. It seems that the other product code number provided - ab619603 - is inco...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録