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Recombinant full length protein corresponding to Human Creatine kinase B type.
Our Abpromise guarantee covers the use of ab71944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Creatine kinase B type knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SHSY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab71944 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab71944 was shown to specifically react with Creatine kinase B type when Creatine kinase B type knockout samples were used. Wild-type and Creatine kinase B type knockout samples were subjected to SDS-PAGE. ab71944 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab71944 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"