Anti-Creatine kinase B type 抗体 [3A6] (ab71944)

製品の概要

  • 製品名Anti-Creatine kinase B type antibody [3A6]
    Creatine kinase B type 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3A6] to Creatine kinase B type
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Creatine kinase B type.

  • ポジティブ・コントロール
    • Human Creatine Kinase MB protein. Recombinant Creatine Kinase BB protein. Recombinant Creatine Kinase MB protein.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 配列類似性Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine phosphokinase BB antibody
    • KCRB_HUMAN antibody
    see all

Anti-Creatine kinase B type antibody [3A6] 画像



  • Predicted band size : 43 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Creatine kinase B type knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: SHSY5Y cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab71944 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab71944 was shown to specifically react with Creatine kinase B type when Creatine kinase B type knockout samples were used. Wild-type and Creatine kinase B type knockout samples were subjected to SDS-PAGE. ab71944 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Creatine kinase B type antibody [3A6] (ab71944) at 1/2000 dilution

    Lane 1 : Recombinant CK-MM protein isolated from E. coli
    Lane 2 : Recombinant CK-BB protein isolated from E. coli
    Lane 3 : Recombinant CK-MB protein isolated from E. coli
    Lane 4 : Human CK-MB protein


    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

Anti-Creatine kinase B type antibody [3A6] (ab71944) 使用論文

ab71944 has not yet been referenced specifically in any publications.

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