Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue, Tissue Homogenate
- Sensitivity: 1 mU/well
製品の概要
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製品名
Creatine Kinase Activity Assay Kit (Colorimetric) -
検出方法
Colorimetric -
サンプルの種類
Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate -
アッセイタイプ
Enzyme activity -
検出感度
< 1 mU/well -
検出範囲
2 nmol/well - 10 nmol/well -
全工程の試験時間
1h 0m -
種交差性
交差種: Mammals, Other species -
製品の概要
Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.
In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.
This assay kit can detect CK activity to less than 1 mU.
Creatine kinase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37ºC -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K777 Creatine Kinase (CK) Activity Colorimetric Assay Kit. K777-100 is the same size as the 100 test size of ab155901.
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Active Creatine Kinase 1 vial Assay Buffer XX 1 x 25ml ATP I 1 vial CK Enzyme Mix 1 vial Creatine 1 x 1ml Developer Solution III 1 vial NADH Standard I 1 vial -
研究分野
画像
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Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
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Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml. -
Typical NADH standard calibration curve using colorimetric reading.
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Creatine kinase activity tested in 5 µl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab155901 は 46 報の論文で使用されています。
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- Younis NS ß-Caryophyllene Ameliorates Cyclophosphamide Induced Cardiac Injury: The Association of TLR4/NF?B and Nrf2/HO1/NQO1 Pathways. J Cardiovasc Dev Dis 9:N/A (2022). PubMed: 35621844
- Boulinguiez A et al. NR1D1 controls skeletal muscle calcium homeostasis through myoregulin repression. JCI Insight 7:N/A (2022). PubMed: 35917173
- Kalinich JF et al. Oxidative damage in metal fragment-embedded Sprague-Dawley rat gastrocnemius muscle. Curr Res Toxicol 3:100083 (2022). PubMed: 35935915
- Yang J et al. Synergetic protective effect of remote ischemic preconditioning and prolyl 4-hydroxylase inhibition in ischemic cardiac injury. Mol Med Rep 25:N/A (2022). PubMed: 35029283