Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339)


  • 製品名Creatine Assay Kit (Colorimetric/Fluorometric)
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • アッセイタイプQuantitative
  • 検出感度
    > 0.001 mM
  • 検出範囲
    0.001 mM - 10 mM
  • 全工程の試験時間
    1h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (?max = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10 mM Creatine.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.

  • アプリケーション適用あり: Functional Studiesmore details


  • 関連性Creatine is an endogenous amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine is generally found as its phosphorylated form phosphocreatine. Creatine functions as part of the cell's energy shuttle: the high energy phosphate group of ATP is transferred to creatinine to form phosphocreatinine in the following reaction: Cr + ATP ? P-Cr + ADP. This reaction is reversibly catalized by creatine kinase. In the human body, creatine is synthesized mainly in the liver from arginine, glycine and methionine. Creatine is excreted as creatinine in the urine.



Our Abpromise guarantee covers the use of ab65339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Creatine Assay Kit (Colorimetric/Fluorometric) 画像

  • Creatine measured in cell lysates showing quantity (nmol) per 106 cells

  • Creatine measured in biologicals showing concentration (micromolar)

  • Assay standard curves: 100 µM top concentration 1 in 2 serial dilutions in duplicate. Analysis was carried out according to ab65339 protocol. To determine the limit and range of detection concentration, the log of concentration was plotted (see standard curves above).

    Limit of detection, absorbance (570 nm): range approx. 1.5 µM – 0.2 µM, excitation/emission (538nm/587nm): range approx.100 µM – 12.5 µM.


Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) 使用論文

ab65339 has not yet been referenced specifically in any publications.

Product Wall

It is correct that this kit can detect phosphorylated and non-phosphorylated creatine.

Sorry for any confusion here.
In the assay, creatine is enzymatically converted to sarcosine which is catalyzed by creatinase. This enzyme works at the first step of the detection reaction in the kit. This conversion event is not dependent on phos...

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Thank you for your enquiry and interest in our products.

Generally, most plate readers have flexibility in their bandwidth of detection. This is generally in terms of ±10, ±20 and so on. If your machine has the flexibility of at...

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Perdona por la espera.

He vuelto a comentar el caso con mis compañeros. Me temo que no tienen ninguna sugerencia que hacer respecto a un control positivo de Creatina para usar con el kit. Podríais utilizar Creatina pura en disti...

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Thank you for contacting us.

I have contacted the lab on your behalf. This product will work very well with mouse samples.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ad...

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Thank you for your phone call.

I can confirm that this kit will not be able to distinguish between phospho-creatine and creatine molecules. The enzyme used in this kit Creatine Amidinohydrolase which converts creatine into Sarcosine.


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Thank you for contacting us.
I have to confirm that we have not specifically tested with this sample type (tissue) and it is therefore not covered by our guarantee.
This kit should be compatible with all mammalian samples and therefore should...

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Thank you for contacting us.

The lab let me know the following:

The assay buffer composition is proprietary information. However, we recommend to use the buffer which comes with each kit, instead of substituting it with another kit...

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Thank you for your inquiry.

The ab65339 kit measures creatine, while the ab65340 kit measures creatinine.

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This product has an expiration date of 6 months from date of arrival if stored at the recommended stated on the datasheet (-20C).

I hope this information is helpful to you. Please do not hesitate to cont...

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