Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.001 mM
製品の概要
-
製品名
Creatine Assay Kit (Colorimetric/Fluorometric) -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate -
アッセイタイプ
Quantitative -
検出感度
> 0.001 mM -
検出範囲
0.001 mM - 10 mM -
全工程の試験時間
1h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples.
In the creatine assay protocol, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (λmax = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10 mM Creatine.
Creatine assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 60 min
- analyze with a microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K635 Creatine Colorimetric/Fluorometric Assay Kit. K635-100 is the same size as the 100 test size of ab65339.
Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.
-
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer II 1 x 25ml Creatinase 1 vial Creatine Standard 1 vial OxiRed Probe 1 x 200µl Sarcosine Enzyme Mix 1 vial -
研究分野
-
関連性
Creatine is an endogenous amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine is generally found as its phosphorylated form phosphocreatine. Creatine functions as part of the cell's energy shuttle: the high energy phosphate group of ATP is transferred to creatinine to form phosphocreatinine in the following reaction: Cr + ATP ? P-Cr + ADP. This reaction is reversibly catalized by creatine kinase. In the human body, creatine is synthesized mainly in the liver from arginine, glycine and methionine. Creatine is excreted as creatinine in the urine.
関連製品
-
Related Products
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab65339 は 9 報の論文で使用されています。
- Ryan CR et al. Creatine Monohydrate Supplementation Increases White Adipose Tissue Mitochondrial Markers in Male and Female Rats in a Depot Specific Manner. Nutrients 13:N/A (2021). PubMed: 34371916
- Sibomana I et al. Urinary Metabolites as Predictors of Acute Mountain Sickness Severity. Front Physiol 12:709804 (2021). PubMed: 34588992
- Peng M et al. Tumour-derived small extracellular vesicles suppress CD8+ T cell immune function by inhibiting SLC6A8-mediated creatine import in NPM1-mutated acute myeloid leukaemia. J Extracell Vesicles 10:e12168 (2021). PubMed: 34807526
- Di Biase S et al. Creatine uptake regulates CD8 T cell antitumor immunity. J Exp Med 216:2869-2882 (2019). PubMed: 31628186
- Wan ES et al. Metabolomic profiling in a Hedgehog Interacting Protein (Hhip) murine model of chronic obstructive pulmonary disease. Sci Rep 7:2504 (2017). PubMed: 28566717
- Fazelzadeh P et al. The Muscle Metabolome Differs between Healthy and Frail Older Adults. J Proteome Res 15:499-509 (2016). PubMed: 26732810
- Côté-Robitaille MÉ et al. Oral supplementations of betaine, choline, creatine and vitamin B6 and their influence on the development of homocysteinaemia in neonatal piglets. J Nutr Sci 4:e31 (2015). PubMed: 26495122
- Rawson ES et al. Low-dose creatine supplementation enhances fatigue resistance in the absence of weight gain. Nutrition 27:451-5 (2011). PubMed: 20591625
- Kumar S et al. The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field. Clinics (Sao Paulo) 66:1237-45 (2011). PubMed: 21876981