製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CRBN
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 35-84 (DQDSKEAKKP NIINFDTSLP TSHTYLGADM EEFHGRTLHD DDSCQVIPVL) of Human CRBN (NP_057386).

  • ポジティブ・コントロール
    • COLO205 cell IF/ICC: SKNSH cell line

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
  • 組織特異性
    Widely expressed. Highly expressed in brain.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • 配列類似性
    Belongs to the CRBN family.
    Contains 1 Lon domain.
  • 翻訳後修飾
    Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
  • 細胞内局在
    Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610203G15Rik antibody
    • 2900045O07Rik antibody
    • AF229032 antibody
    • AW108261 antibody
    • Cereblon antibody
    • Crbn antibody
    • CRBN_HUMAN antibody
    • DKFZp781K0715 antibody
    • MGC27358 antibody
    • MRT2A antibody
    • OTTHUMP00000209555 antibody
    • piL antibody
    • Protein cereblon antibody
    • Protein x 0001 antibody
    see all

Anti-CRBN antibody 画像

  • Anti-CRBN antibody (ab98992) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Predicted band size : 50 kDa
  • ICC/IF image of ab98992 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98992, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CRBN antibody (ab98992) 使用論文

This product has been referenced in:
  • Min Y  et al. Cereblon negatively regulates TLR4 signaling through the attenuation of ubiquitination of TRAF6. Cell Death Dis 7:e2313 (2016). Read more (PubMed: 27468689) »
  • Gandhi AK  et al. Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity. Br J Haematol 164:233-44 (2014). WB . Read more (PubMed: 24206017) »

See all 2 Publications for this product

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Expiration date: May 30th, 2012

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Read More
Abreviews
Application
Western blot
Sample
Mouse Purified protein (Purified protein of HEK293(mouse CRBN-His6 overexp)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
3 µg
Specification
Purified protein of HEK293(mouse CRBN-His6 overexp
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 30 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Myeloma cell)
Specification
Myeloma cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

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Verified customer

投稿 Feb 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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