製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to CRBN
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein corresponding to Human CRBN aa 1-442.
    Sequence:

    MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFD TSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQ LFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDF GIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLE SLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDA ETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLK IGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYV NPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGW KFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL


    Database link: Q96SW2

  • ポジティブ・コントロール
    • WB: CRBN transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ターゲット情報

  • 機能
    Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
  • 組織特異性
    Widely expressed. Highly expressed in brain.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • 配列類似性
    Belongs to the CRBN family.
    Contains 1 Lon domain.
  • 翻訳後修飾
    Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
  • 細胞内局在
    Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2610203G15Rik antibody
    • 2900045O07Rik antibody
    • AF229032 antibody
    • AW108261 antibody
    • Cereblon antibody
    • Crbn antibody
    • CRBN_HUMAN antibody
    • DKFZp781K0715 antibody
    • MGC27358 antibody
    • MRT2A antibody
    • OTTHUMP00000209555 antibody
    • piL antibody
    • Protein cereblon antibody
    • Protein x 0001 antibody
    see all

Anti-CRBN antibody 画像

  • All lanes : Anti-CRBN antibody (ab68763) at 1/500 dilution

    Lane 1 : CRBN transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate


    Predicted band size : 51 kDa

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

プロトコール

Anti-CRBN antibody (ab68763) 使用論文

ab68763 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Purified protein (Purified protein of HEK293(mouse CRBN-His6 overexp)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
3 µg
Specification
Purified protein of HEK293(mouse CRBN-His6 overexp
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 30 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Treatment
CRBN Overexpression
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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