製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CRABP2
  • 特異性
    ab74365 detects endogenous levels of total CRABP2 protein.
  • アプリケーション
    適用あり: ICC, WB, Indirect ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human CRABP2.

  • ポジティブ・コントロール
    • Extracts from HT-29 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 16 kDa.
Indirect ELISA 1/20000.
IHC-P 1/100.

ターゲット情報

  • 機能
    Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
  • 配列類似性
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメイン
    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 細胞内局在
    Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cellular retinoic acid binding protein 2 antibody
    • Cellular retinoic acid binding protein II antibody
    • Cellular retinoic acid-binding protein 2 antibody
    • Cellular retinoic acid-binding protein II antibody
    • CRABP II antibody
    • CRABP-II antibody
    • Crabp2 antibody
    • CRABPII antibody
    • RABP2_HUMAN antibody
    • RBP6 antibody
    • Rretinoic acid-binding protein, cellular, type II antibody
    see all

画像

  • All lanes : Anti-CRABP2 antibody (ab74365) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from HT-29 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • ab74365 staining CRABP2 in Human Schwann Cells by ICC (Immunocytochemistry). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 25°C. An Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Yao PL  et al. Activation of peroxisome proliferator-activated receptor-ß/d (PPAR-ß/d) inhibits human breast cancer cell line tumorigenicity. Mol Cancer Ther 13:1008-17 (2014). Human . Read more (PubMed: 24464939) »
  • Morris DR & Levenson CW Zinc regulation of transcriptional activity during retinoic acid-induced neuronal differentiation. J Nutr Biochem 24:1940-4 (2013). WB ; Human . Read more (PubMed: 24029070) »

See all 3 Publications for this product

レビューと Q&A

Thank you for contacting us.

The immuogen is from amino acid 50 - 100 of human CRABP2; http://www.uniprot.org/uniprot/P29373

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice o...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Schwann Cell (T265))
Specification
Schwann Cell (T265)
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton/PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adult testis)
Specification
adult testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer
Permeabilization
Yes - 0.1% Triton-100 in 1x PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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