製品の概要

  • 製品名
    Anti-CPT2 antibody [1C2AE6]
    CPT2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1C2AE6] to CPT2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, ICC/IF, Flow Cyt, IP, In-Cell ELISAmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Human liver mitochondria

  • ポジティブ・コントロール
    • HepG2 cells. HL-60 cells. Human normal colon FFPE tissue
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 4 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 4 µg/ml.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • パスウェイ
      Lipid metabolism; fatty acid beta-oxidation.
    • 関連疾患
      Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency (CPT2D) [MIM:255110, 600649]; also known as CPT-II deficiency or CPT2 deficiency. CPT2D is an autosomal recessive disorder characterized by recurrent myoglobinuria, episodes of muscle pain, stiffness, and rhabdomyolysis. These symptoms are triggered by prolonged exercise, fasting or viral infection and patients are usually young adults. In addition to this classical, late-onset, muscular type, a hepatic or hepatocardiomuscular form has been reported in infants. Clinical pictures in these children or neonates include hypoketotic hypoglycemia, liver dysfunction, cardiomyopathy and sudden death.
      Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency, lethal neonatal (CPT2D-LN) [MIM:608836]; also known as lethal neonatal CPT-II deficiency. It is a lethal neonatal form of CPT2D. This rarely presentation is antenatal with cerebral periventricular cysts and cystic dysplastic kidneys. The clinical variability of the disease is likely attributed to the variable residual enzymatic activity.
    • 配列類似性
      Belongs to the carnitine/choline acetyltransferase family.
    • 細胞内局在
      Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Carnitine O palmitoyltransferase 2 antibody
      • Carnitine O palmitoyltransferase 2 mitochondrial antibody
      • Carnitine O-palmitoyltransferase 2 antibody
      • Carnitine palmitoyltransferase 2 antibody
      • Carnitine palmitoyltransferase II antibody
      • CPT 1 antibody
      • CPT 2 antibody
      • CPT II antibody
      • CPT1 antibody
      • CPT2 antibody
      • CPT2_HUMAN antibody
      • CPTASE antibody
      • CPTII antibody
      • IIAE4 antibody
      • mitochondrial antibody
      see all

    画像

    • Immunocytochemistry image of ab110293 stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110293 at 4 µg/ml for 2h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
    • HL-60 cells were stained with 1 µg/mL of ab110293 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
    • SDS-PAGE analysis of IP samples. As an IP antibody, ab110293 works with human, bovine, rat, and mouse samples.

      Lane STD: Molecular weight ladder

      Lane HLM: Human liver mitochondria

      Lane BHM: Bovine heart mitochondria

      Lane RLM: Rat liver mitochondria

      Lane MLM: Mouse liver mitochondria

    • IHC image of CPT2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110293, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献

    This product has been referenced in:
    • Townsend KL  et al. Increased Mitochondrial Activity in BMP7-Treated Brown Adipocytes, Due to Increased CPT1- and CD36-Mediated Fatty Acid Uptake. Antioxid Redox Signal : (2012). Read more (PubMed: 22938691) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Treatment
    Wild type and Knockout mouse tissue
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 02 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録