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Our Abpromise guarantee covers the use of ab53532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).|
|IHC-P||Use a concentration of 4 - 6 µg/ml.|
ab53532 at 4µg/ml staining CPT1A in human kidney tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was successfully used.