製品の概要

  • 製品名Anti-CPSF73 antibody
    CPSF73 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CPSF73
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residue 1 and 50 of human CPSF73 (NP_057291.1)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa, 293T and mouse NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
    • 配列類似性Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
    • 翻訳後修飾Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cleavage and polyadenylation specific factor 3 73kDa antibody
      • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 3 antibody
      • CPSF 73 antibody
      • CPSF 73 kDa subunit antibody
      • CPSF antibody
      • CPSF-73 antibody
      • cpsf3 antibody
      • CPSF3_HUMAN antibody
      • CPSF73 antibody
      • mRNA 3''-end-processing endonuclease CPSF-73 antibody
      • YSH1 antibody
      see all

    Anti-CPSF73 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CPSF73 with ab72294 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-CPSF73 antibody (ab72294) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 77 kDa
      Observed band size : 77 kDa


      Exposure time : 3 minutes

    Anti-CPSF73 antibody (ab72294) 使用論文

    ab72294 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab72294.
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