製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CPSF2
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
  • 免疫原

    Synthetic peptide designed between residues 732 and 782 of Human CPSF2 (NP_059133.1)

  • ポジティブ・コントロール
    • HeLa, 293T or NIH3T3 whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性
    CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • Cleavage and polyadenylation specific factor 2 antibody
    • cleavage and polyadenylation specific factor 2, 100kDa antibody
    • Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 2 antibody
    • CPSF 100 kDa subunit antibody
    • CPSF 2 antibody
    • CPSF100 antibody
    • KIAA1367 antibody
    • zgc:92484 antibody
    see all

画像

  • All lanes : Anti-CPSF2 antibody (ab81554) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 seconds
  • HeLa whole cell lysate (1mg) was immunoprecipitated with ab81554 at 3µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81554 at 1µg/ml.

参考文献

ab81554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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