製品の概要

  • 製品名Anti-CPSF1 antibody
    CPSF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CPSF1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: GCYDMWTVIA PVRKEEEDNP KGEGTEQEPS TTPEADDDGR RHGFLILSRE, corresponding to amino acids 531-580 of Human CPSF1

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 144 kDa (predicted molecular weight: 161 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 関連性CPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
  • 細胞内局在Nucleus, nucleoplasm.
  • 参照データベース
  • 別名
    • Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 1 antibody
    • CPSF 160 kDa subunit antibody
    • CPSF160 antibody
    • HSU37012 antibody
    • P/cl.18 antibody
    see all

Anti-CPSF1 antibody 画像

  • Anti-CPSF1 antibody (ab62598) at 0.5 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 161 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)

Anti-CPSF1 antibody (ab62598) 使用論文

ab62598 has not yet been referenced specifically in any publications.

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