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Full length native protein (purified) (Rat).
Our Abpromise guarantee covers the use of ab3682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ab3682 staining CPS1 in mouse hepatic tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in Citrate buffer and then blocked in maleate blocking solution for 30 minutes at 22°C. The primary antibody was diluted 1/500 and incubated with sample in maleate buffer blocking solution for 16 hours at 22°C. A Biotin conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/250 as secondary antibody.
ab3682 staining CPS1 in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 100% maleate blocking solution at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) at 220C for 16 hours. An Alexa Fluor®488-conjugated donkey polyclonal to rabbit IgG was used undiluted as a secondary antibody. The image demonstrates CPS1 in green, nuclei stained blue with DAPI.
CPS-1 localisation is consistent with the reticulated pattern of liver mitonchondria.
Rabbit polyclonal to CPS-1 (ab3682), at 1/5000 on COS-7 cells transiently transfected with pCMV5-CPS 1 plasmid (Corvi, M.M and Berthiaume, L.G. unpublished data).
Secondary: donkey anti-rabbit (Texas Red).