Rabbit polyclonal to CPNE1
Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide: ETVQKLRFGI YDIDNKTPEL RDDDFLGGAE CSLGQIVSSQ VLTLPLMLKP, corresponding to amino acids 74-123 of Human CPNE1
HepG2 cell lysate. Human intestine.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
May function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties.
Belongs to the copine family.
Contains 2 C2 domains. Contains 1 VWFA domain.
Information by UniProt
Copine 1 antibody
Copine I antibody
Western blot - CPNE1 antibody (ab66898)
All lanes :
Anti-CPNE1 antibody (ab66898) at 5 µg/ml (in 5% skim milk / PBS buffer)
Lane 1 :
Molecular weight markers
Lane 2 :
HepG2 cell lysate at 10 µg
Secondary All lanes :
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
57 kDa (
why is the actual band size different from the predicted?
Additional bands at:
25 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CPNE1 antibody (ab66898)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling CPNE1 with ab66898 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.
has not yet been referenced specifically in any publications.
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