Anti-CPI17 alpha (phospho T38) 抗体 (ab52174)

製品の概要

  • 製品名
    Anti-CPI17 alpha (phospho T38) antibody
    CPI17 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CPI17 alpha (phospho T38)
  • 特異性
    ab52174 detects endogenous levels of CPI17a only when phosphorylated at threonine 38.
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human CPI17a around the phosphorylation site of threonine 38 (R-V-TP-V-K).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab52174 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

ターゲット情報

  • 機能
    Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
  • 組織特異性
    Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
  • 配列類似性
    Belongs to the PP1 inhibitor family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
    • 17-KDa protein antibody
    • 17kDa PKC potentiated inhibitory protein of PP1 antibody
    • CPI 17 alpha antibody
    • CPI 17 antibody
    • CPI 17alpha antibody
    • CPI-17 antibody
    • CPI17 antibody
    • PKC potentiated inhibitory protein of PP1 antibody
    • PKC potentiated inhibitory protein of PP1, 17-KD antibody
    • PP14A_HUMAN antibody
    • PPP1INL antibody
    • Ppp1r14a antibody
    • Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD antibody
    • Protein kinase C-potentiated inhibitor protein of 17 kDa antibody
    • Protein phosphatase 1 antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 14A antibody
    • Protein phosphatase 1 regulatory subunit 14A antibody
    • Regulatory subunit 14A antibody
    see all

画像

  • ab52174 at 1/50 dilution staining CPI17a in human breast carcinoma by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunising peptide.


  • Predicted band size : 17 kDa

参考文献

This product has been referenced in:
  • Kang S  et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . Read more (PubMed: 24449418) »

See 1 Publication for this product

レビューと Q&A

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells. Read More

Thank you for contacting us.
This antibody has been validated for Western blot using RAW264.7 cell lysate. It has not been tested against other mouse cell lines, such as C2C12.


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Myometrial smooth muscle cells)
Loading amount
15 µg
Specification
Myometrial smooth muscle cells
Treatment
1 or 10 nM oxytocin for 1 minute, and 1µM phorbol 12-myristate 13-acetate for 10 minutes
Gel Running Conditions
Reduced Denaturing (13% tris-glycine SDS-PAGE gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Apr 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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