製品の概要

  • 製品名Anti-CPI17 alpha antibody [E305]
    CPI17 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E305] to CPI17 alpha
  • 特異性ab32213 recognises CPI17 alpha and may detect isoform CPI17 beta.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CPI17 alpha aa 1-100 (N terminal).

  • エピトープab32213 reacts with an epitope located in the N terminal region of CPI17 alpha
  • ポジティブ・コントロール
    • Hela, Jurkat, MCF-7 and A431 cell lysate
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名E305
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP 1/80.
  • 追加情報Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
    • 組織特異性Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
    • 配列類似性Belongs to the PP1 inhibitor family.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
      • 17-KDa protein antibody
      • 17kDa PKC potentiated inhibitory protein of PP1 antibody
      • CPI 17 alpha antibody
      • CPI 17 antibody
      • CPI 17alpha antibody
      • CPI-17 antibody
      • CPI17 antibody
      • PKC potentiated inhibitory protein of PP1 antibody
      • PKC potentiated inhibitory protein of PP1, 17-KD antibody
      • PP14A_HUMAN antibody
      • PPP1INL antibody
      • Ppp1r14a antibody
      • Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD antibody
      • Protein kinase C-potentiated inhibitor protein of 17 kDa antibody
      • Protein phosphatase 1 antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 14A antibody
      • Protein phosphatase 1 regulatory subunit 14A antibody
      • Regulatory subunit 14A antibody
      see all

    Anti-CPI17 alpha antibody [E305] 画像

    • Lanes 1, 2 & 4 : purified at 1/1000 dilution
      Lane 3 : RAW264.7 purified at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CPI17 alpha antibody [E305] (ab32213) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • ab32213 (purified) at 1/50 immunoprecipitating CPI17 in MCF7 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-CPI17 alpha antibody [E305] (ab32213) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-CPI17 alpha antibody [E305] (ab32213) at 1/5000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : A431 cell lysate


      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • ab32213 (unpurified) at 1/50 immunoprecipitating CPI17 in MCF7 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-CPI17 alpha antibody [E305] (ab32213) 使用論文

    This product has been referenced in:
    • Kang S  et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . Read more (PubMed: 24449418) »
    • Lima VV  et al. Vascular O-GlcNAcylation augments reactivity to constrictor stimuli by prolonging phosphorylated levels of the myosin light chain. Braz J Med Biol Res 47:826-33 (2014). WB ; Rat . Read more (PubMed: 25140811) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"