製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CPEB4
  • アプリケーション
    適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to N terminal amino acids 72-121 (DEILGSEKAK SQQQEQQDPL EKQQLSPSPG QEAGILPETE KAKSEENQGD) of human CPEB4 (NP_085130).

  • ポジティブ・コントロール
    • Fetal lung lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-CPEB4 antibody 画像

  • Anti-CPEB4 antibody (ab83009) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-CPEB4 antibody (ab83009) 使用論文

This product has been referenced in:
  • Guillén-Boixet J  et al. CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. Elife 5:N/A (2016). Read more (PubMed: 27802129) »
  • Pérez-Guijarro E  et al. Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Nat Commun 7:13418 (2016). WB ; Human . Read more (PubMed: 27857118) »

See all 2 Publications for this product

Product Wall

Thank you for your email. I am sorry for all the inconvenience. I am still expecting an answer from the laboratory. As requested, I have issued a free of charge replacement with one vial of ab83009 lot x with the order number x. The vial should arrive ...

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Thank you for calling us and for alerting usof the problem you are experiencing withthe new batch of this antibody. We take product complaints very seriously, and investigate every product and batch that we feel may not be performing correctly.
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Muchas gracias por haberte tomado la molestia de enviarnos el cuestionario y las imágenes adjuntas.

Efectivamente, comparando las imágenes de los dos lotes, el último no está dando la señal esperada. He veri...

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Gracias por tu respuesta y por confirmar los detalles del pedido.

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo ab83009.

Recibiréis una confirmación automática del mismo con más de...

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Muchas gracias por contactarnos y alertarnos del exceso de volumen recibido.

He contactado con mis compañeros encargados de alicuotar los anticuerpos, y me han comentado que normalmente suele haber un exceso de volumen de anticuerpo pa...

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Amir Djourabchi

Verified customer

投稿 Apr 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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