製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報Peptide ELISA: antibody detection limit dilution 1:128,000
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 65 kDa. (65-70kDa band observed in Human Brain lysates. Please note this band was always accompanied by a slightly stonger band at approx. 48kDa and both bands could be blocked by incubation with the immunising peptide).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
    • 組織特異性Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
    • 配列類似性Belongs to the RRM CPEB family.
      Contains 2 RRM (RNA recognition motif) domains.
    • ドメインThe 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
    • 翻訳後修飾Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
    • 細胞内局在Cytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CEBP antibody
      • CPE binding protein 1 antibody
      • CPE BP1 antibody
      • CPE-binding protein 1 antibody
      • CPE-BP1 antibody
      • CPEB 1 antibody
      • CPEB antibody
      • CPEB-1 antibody
      • CPEB1 antibody
      • CPEB1 protein antibody
      • CPEB1_HUMAN antibody
      • Cytoplasmic polyadenylation binding protein 1 antibody
      • Cytoplasmic polyadenylation element binding protein antibody
      • Cytoplasmic polyadenylation element binding protein 1 antibody
      • Cytoplasmic polyadenylation element-binding protein 1 antibody
      • FLJ13203 antibody
      • h CEBP antibody
      • h-CEBP antibody
      • hCPEB 1 antibody
      • hCPEB-1 antibody
      • MGC34136 antibody
      • MGC60106 antibody
      • mKIAA0940 antibody
      • MKIAA0940 protein antibody
      see all

    Anti-CPEB1 antibody 画像

    • Anti-CPEB1 antibody (ab15917) at 1/0.5 dilution + Human Brain lysate at 35 µg

      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 49 kDa (possible cross reactivity, but this band is not blocked).

      Exposure time : 1 hour

    Anti-CPEB1 antibody (ab15917) 使用論文

    ab15917 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab15917.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"