Anti-Coxsackie Adenovirus Receptor 抗体 (ab100811)

製品の概要

  • 製品名Anti-Coxsackie Adenovirus Receptor antibody
    Coxsackie Adenovirus Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Coxsackie Adenovirus Receptor
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 335-365 of Human Coxsackie Adenovirus Receptor (NP_001329.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
  • 組織特異性Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
  • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメインThe Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
  • 翻訳後修飾N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
  • 細胞内局在Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Information by UniProt
  • 参照データベース
  • 別名
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    • Coxsackievirus B adenovirus receptor antibody
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    • CVB3-binding protein antibody
    • Cxadr antibody
    • CXAR_HUMAN antibody
    • hCAR antibody
    • HCVADR antibody
    see all

Anti-Coxsackie Adenovirus Receptor antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Coxsackie Adenovirus Receptor with ab100811 at 1/1000 (1µg/ml). Detection: Peroxidase Substrate.
  • All lanes : Anti-Coxsackie Adenovirus Receptor antibody (ab100811) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg


    Predicted band size : 40 kDa


    Exposure time : 3 minutes
  • ab100811 at 0.4 µg/ml staining Coxsackie Adenovirus Receptor in HeLa cell lysate immunoprecipitated using ab100811 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds

Anti-Coxsackie Adenovirus Receptor antibody (ab100811) 使用論文

ab100811 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Mouse Tissue lysate - whole (Testis)
Specification Testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Aug 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"