製品の概要

  • 製品名
    Anti-COX6A1 antibody [14A3AD2BH4]
    COX6A1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14A3AD2BH4] to COX6A1
  • 特異性
    ab110265 recognises the Liver isoform in human, rat, mouse.
  • アプリケーション
    適用あり: Flow Cyt, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide:

    SSGAHGEEGSARMWKALTLF(C)

    , corresponding to amino acids 25-44 of Cow COX6A1

  • ポジティブ・コントロール
    • HepG2, Human, Mouse and Rat heart lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • 特記事項(精製)
    ab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • ポリ/モノ
    モノクローナル
  • クローン名
    14A3AD2BH4
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 12 kDa.

ターゲット情報

  • 機能
    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • 配列類似性
    Belongs to the cytochrome c oxidase subunit 6A family.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COX VIa L antibody
    • COX VIa-L antibody
    • COX6A antibody
    • COX6A1 antibody
    • COX6AL antibody
    • CX6A1_HUMAN antibody
    • Cytochrome c oxidase polypeptide VIa liver antibody
    • Cytochrome c oxidase polypeptide VIa-liver antibody
    • Cytochrome c oxidase subunit 6A1 antibody
    • Cytochrome c oxidase subunit 6A1 mitochondrial antibody
    • Cytochrome C oxidase subunit VIa homolog antibody
    • Cytochrome c oxidase subunit VIA liver antibody
    • Cytochrome c oxidase subunit VIa polypeptide 1 antibody
    • Cytochrome c oxidase subunit VIA-liver antibody
    • MGC104500 antibody
    • mitochondrial antibody
    see all

画像

  • Overlay histogram showing HepG2 cells stained with ab110265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110265, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-COX6A1 antibody [14A3AD2BH4] (ab110265) at 5 µg/ml

    Lane 1 : Human Heart Lysate
    Lane 2 : Rat Heart Lysate
    Lane 3 : Mouse Heart Lysate
    Lane 4 : HepG2 cell lysate


    Predicted band size : 12 kDa
    Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

参考文献

This product has been referenced in:
  • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). Read more (PubMed: 18538373) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (brain mito)
Gel Running Conditions
Non-reduced Denaturing (NuPAGE 4-12%)
Loading amount
20 µg
Specification
brain mito
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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