製品の概要

  • 製品名
    Anti-COX5A antibody [6E9B12D5]
    COX5A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6E9B12D5] to COX5A
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Zebrafish
  • 免疫原

    Cow COX5A

  • ポジティブ･コントロール
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • 特記事項（精製）
    ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • ポリ/モノ
    モノクローナル
  • クローン名
    6E9B12D5
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 22748147
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

画像

  • Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Western blot - Anti-COX5A antibody [6E9B12D5] (ab110262)
    All lanes : Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg

    Predicted band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).
  • Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)
    Flow Cytometry - Anti-COX5A antibody [6E9B12D5] (ab110262)
    HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

参考文献

This product has been referenced in:
  • Nam M  et al. Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression. Sci Rep 7:2013 (2017). Read more (PubMed: 28515438) »
  • Gómez-Serrano M  et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). Human . Read more (PubMed: 28064117) »

See all 36 Publications for this product

ab110262 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

4 reviews or Q&A

10
Votes: Highest First

Thank you, we look forward to receiving the replacement. The lot you have available, GR47109-2, will be fine.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab110261.
To check the status of the order please contact our Cu...

Read More

Dear Technical Support/Custom Service,



We purchased ab110262 on March 26, 2012, and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitution/replacement (MS408...

Read More

Thanks for sending the western blot data to my Outlook account. I agree it is very strange that the powerpoint file somehow turned into one of our datasheets!
I agree that the vial of ab110262 you received is not working as expcted. I am happy to ...

Read More

Please see the attached image of another experiment with a negative
result that we obtained with Ab110262 COX5a lot GR73589-1. Moreover, we don't
need to strip the membranes prior to the second blot (with CORE2), since
the two p...

Read More

Thanks for your reply.
Oddly, the powerpoint attachement opens up to show an image of one of our datasheets, for ab110262. Not sure what the cause is but if you could, please forward the powerpoint image to my outlook account.

Dear Technical Support/Custom Service,



We purchased ab110262 on March 26, 2012 (Order No. 1059903), and unfortunately it did not perform in our experiments. Please see attached image. Would it be possible to receive a substitutio...

Read More

Thank you for your enquiry. I am sorry to hear this vial of ab110262 is giving you trouble.
And thanks for including the western blot images. If I understand you correctly, you first probed the membrane with anti-CORE2 antibody and then probed the...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録