Anti-COX2 / Cyclooxygenase 2 抗体 [SP21], prediluted (ab21704)

製品の概要

  • 製品名Anti-COX2 / Cyclooxygenase 2 antibody [SP21], prediluted
    COX2 / Cyclooxygenase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2, prediluted
  • 特異性ab21704 recognises COX2 (Cyclooxygenase 2).
  • アプリケーション適用あり: IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to C terminus of human COX2.

  • ポジティブ・コントロール
    • Lung carcinoma, colon carcinoma, breast carcinoma.

アプリケーション

Our Abpromise guarantee covers the use of ab21704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/1.
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

ターゲット情報

  • 機能Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • パスウェイLipid metabolism; prostaglandin biosynthesis.
  • 配列類似性Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻訳後修飾S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 細胞内局在Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody [SP21], prediluted 画像

  • ab21704 staining COX2 / Cyclooxygenase 2 in murine spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 4% paraformaldehyde, blocked with 10% goat serum for 30 minutes at 24°C then incubated with neat ab21704 for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution. Counterstained with Hoescht 33342.

    See Abreview

  • ab21704 staining COX2 / Cyclooxygenase 2 in rat spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in formaldehyde, blocked using 10% serum for 30 minutes at 24°C, then incubated with ab21704 for 16 hours at 4°C. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, staining COX2 / Cyclooxygenase 2 with ab21704.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma tissue, staining COX2 / Cyclooxygenase 2 with ab21704.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocarcinoma tissue, staining COX2 / Cyclooxygenase 2 with ab21704.

Anti-COX2 / Cyclooxygenase 2 antibody [SP21], prediluted (ab21704) 使用論文

This product has been referenced in:
  • Nachat-Kappes R  et al. Effects of enriched environment on COX-2, leptin and eicosanoids in a mouse model of breast cancer. PLoS One 7:e51525 (2012). IHC ; Mouse . Read more (PubMed: 23272114) »
  • Wagemakers M  et al. COX-2 Inhibition Combined with Radiation Reduces Orthotopic Glioma Outgrowth by Targeting the Tumor Vasculature. Transl Oncol 2:1-7 (2009). IHC-Fr ; Mouse . Read more (PubMed: 19252746) »

See all 2 Publications for this product

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I would recommend ab15191, as we guarantee it to work on mouse samples in IHC-Frozen samples and we have a number of Abreviews and references that have successfully used this antibody.

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab21704.  Please note that this free of charge replacement vial is also covere...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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Abcam user community

Verified customer

投稿 Aug 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Aug 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"