Anti-COX2 / Cyclooxygenase 2 抗体 [SP21] (ab16701)

製品の概要

  • 製品名Anti-COX2 / Cyclooxygenase 2 antibody [SP21]
    COX2 / Cyclooxygenase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
  • アプリケーション適用あり: IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide (Rat) (C terminal).

  • ポジティブ・コントロール
    • Lung carcinoma, colon carcinoma and breast carcinoma; HT 29 lysate.

アプリケーション

Our Abpromise guarantee covers the use of ab16701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • パスウェイLipid metabolism; prostaglandin biosynthesis.
    • 配列類似性Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • 翻訳後修飾S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • 細胞内局在Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Anti-COX2 / Cyclooxygenase 2 antibody [SP21] 画像

    • ab16701 at 1/100 dilution staining COX2 / Cyclooxygenase 2 in human breast carcinoma by Immunohistochemistry (FFPE-sections).

    Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701) 使用論文

    This product has been referenced in:
    • Fujita M  et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res 71:2664-74 (2011). Read more (PubMed: 21324923) »
    • Lu TC  et al. Analgesic and Anti-Inflammatory Activities of the Methanol Extract from Pogostemon cablin. Evid Based Complement Alternat Med : (2009). Read more (PubMed: 19933324) »

    See all 2 Publications for this product

    Product Wall

    Unfortunately it is not possible to determine the concentration since tissue culture supernatant will contain many other host proteins besides the antibody of interest as it is unpurified. As a rough guide tissue culture supernatant contains 1 mg/ml to...

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    I'm very sorry to hear you are experiencing problems with ab16701. The protocol you have used is very good and should work well; in light of your problems it is clear that the antibody did not work well in western blotting. I would like to offer ...

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    Thank you for your enquiry. I am not sure that I follow your question, please can you elaborate. ab16701, anti-COX2 recognizes both the rat COX2 (69164KDa) and the human COX2 (68996KDa). I hope this information helps. Please do not hesitate...

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    Our source for this antibody has recommended HT 29 lysate as a positive control for Western blotting. This type of lysate can be obtained from Abcam; it is catalog # ab3952. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"