製品の概要

  • 製品名
    Anti-COX2 / Cyclooxygenase 2 antibody
    COX2 / Cyclooxygenase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, Sandwich ELISA, IP, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Syrian hamster
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Rat)

  • ポジティブ・コントロール
    • IHC: Lung carcinoma and colon carcinoma.

アプリケーション

Our Abpromise guarantee covers the use of ab15191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 20660112
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab90345 as Capture.
IP Use at an assay dependent concentration.
IHC-P 1/200 - 1/1000.
WB Use at an assay dependent concentration. Detects a band of approximately 69 kDa.

ターゲット情報

  • 機能
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • パスウェイ
    Lipid metabolism; prostaglandin biosynthesis.
  • 配列類似性
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻訳後修飾
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 細胞内局在
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

画像

  • ab15191 staining COX2 in HepG2 cells treated with sinomenine hydrochloride (ab141190), by ICC/IF. Decrease of COX2 expression correlates with increased concentration of sinomenine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141190 (sinomenine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15191 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml

    Lane 1 : Recombinant human COX2 / Cyclooxygenase 2 protein (ab58868) at 0.1 µg
    Lane 2 : Recombinant human COX2 / Cyclooxygenase 2 protein (ab58868) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • ab15191 staining mouse brain sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin

    See Abreview

  • Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml
  • ab15191 staining COX2 in Human Colorectal Tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA) for 2 hours at 24°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab15191 staining adult rat hippocampus tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT.  The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.

    Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen.  In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.

    See Abreview

  • ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr.  Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C.  A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.

    See Abreview

  • ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).

  • ab15191 staining COX2 / Cyclooxygenase 2 in Mouse liver tissue sections by IHC-Fr.

    Cells were fixed with acetone and blocked with 10% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton x-100) for 13 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Simonsson M  et al. The prognostic impact of COX-2 expression in breast cancer depends on oral contraceptive history, preoperative NSAID use, and tumor size. Int J Cancer 140:163-175 (2017). Read more (PubMed: 27632554) »
  • Tang SH  et al. Expression of cyclooxygenase-2 is correlated with lncRNA-COX-2 in cirrhotic mice induced by carbon tetrachloride. Mol Med Rep 15:1507-1512 (2017). IHC-P ; Mouse . Read more (PubMed: 28259935) »

See all 71 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1(Ventana EDTA)
Permeabilization
No
Specification
Colon
Fixative
10% neutral formalin
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Abcam user community

Verified customer

投稿 Oct 12 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (human brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Treatment
huntington's disease
Specification
human brain
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 May 16 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain (striatum) lysate)
Gel Running Conditions
Reduced Denaturing (4-20% TGX stain free (Tris gly))
Loading amount
20 µg
Specification
Mouse brain (striatum) lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Misha Riley

Verified customer

投稿 May 05 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Abdominal endometriotic lesion)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
15 µg
Specification
Abdominal endometriotic lesion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Mar 21 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (peritonei tumor)
Permeabilization
Yes - 0.3% Triton
Specification
peritonei tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Feb 07 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LS174T)
Permeabilization
Yes - 0.3% TritonX -100
Specification
LS174T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Feb 01 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Tissue sections (Colorectal Tumor)
Specification
Colorectal Tumor
Permeabilization
Yes - 0.3% Triton-X-100
Fixative
Paraformaldehyde
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投稿 Mar 27 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (fibroblast)
Specification
fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
50 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Feb 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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投稿 Feb 06 2013

1-10 of 52 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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