製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to COX10
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide within residues: APGPFDWPCF LLTSVGTGLA SCAANSINQF FEVPFDSNMN RTKNRPLVRG , corresponding to internal sequence amino acids 180-229 of Human COX10 (NP_001294)

  • ポジティブ・コントロール
    • Human fetal lung lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Converts protoheme IX and farnesyl diphosphate to heme O.
  • 関連疾患
    Defects in COX10 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in COX10 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
  • 配列類似性
    Belongs to the ubiA prenyltransferase family.
  • 細胞内局在
    Mitochondrion membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2410004F01Rik antibody
    • AU042636 antibody
    • COX10 antibody
    • COX10_HUMAN antibody
    • Cytochrome c oxidase assembly protein antibody
    • Cytochrome c oxidase subunit X antibody
    • Heme A farnesyltransferase antibody
    • Heme O synthase antibody
    • OTTMUSP00000006085 antibody
    • Protoheme IX farnesyltransferase, mitochondrial antibody
    • Protoheme IX farnesyltransferase, mitochondrial precursor antibody
    • RP23-78H18.1 antibody
    see all

画像

  • ICC/IF image of ab84053 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84053 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-COX10 antibody (ab84053) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • All lanes : Anti-COX10 antibody (ab84053) at 1/1000 dilution

    Lane 1 : Isolated mitochondria from wild type mice at 15 µg
    Lane 2 : Isolated mitochondria from wild type mice at 30 µg


    Predicted band size : 49 kDa

参考文献

ab84053 has not yet been referenced specifically in any publications.

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