製品の概要

  • 製品名
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Urine 60 <=6.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Urine 30 <=7.2%
  • サンプルの種類
    Urine
  • アッセイタイプ
    Competitive
  • 検出感度
    2.95 ng/ml
  • 検出範囲
    10 ng/ml - 500 ng/ml
  • 添加回収試験

    107.48 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.


    A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit
    Cortisol Standard 0 - 0 ng/mL 1 x 1ml
    Cortisol Standard 1 - 10 ng/mL 1 x 1ml
    Cortisol Standard 2 - 50 ng/mL 1 x 1ml
    Cortisol Standard 3 - 150 ng/mL 1 x 1ml
    Cortisol Standard 4 – 500 ng/mL 1 x 1ml
    Cortisol-HRP Conjugate 1 x 33ml
    Cover foils 1 unit
    High control 1 x 1ml
    Low control 1 x 1ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab108674

プロトコール

参考文献

ab108674 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.

This product comes with full money back guarantee if used with human urine samples. Please reassure them if the product does not work they will get refund or replacements.

I would advise them buying 1 prod...

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We do have several kits available against the products requested. However, none of them have unfortunately been tested with fish.

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To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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