製品の概要

  • 製品名
    Anti-Cortisol Binding Globulin antibody
    Cortisol Binding Globulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cortisol Binding Globulin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids 200-229 of Human Cortisol Binding Globulin (NP_001747.2).

  • ポジティブ･コントロール
    • Mouse kidney tissue lysates IHC-P: human liver tissue sections. IF/ICC: HepG2 cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.

ターゲット情報

  • 機能
    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • 組織特異性
    Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.
  • 関連疾患
    Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue.
  • 配列類似性
    Belongs to the serpin family.
  • 翻訳後修飾
    N-glycosylated; binds 5 oligosaccharide chains.
    Glycosylation in position Asn-260 is needed for steroid binding.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CBG antibody
      • CBG_HUMAN antibody
      • corticosteroid binding globulin antibody
      • Corticosteroid-binding globulin antibody
      • serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
      • Serpin A6 antibody
      • serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
      • Serpina6 antibody
      • Transcortin antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Cortisol Binding Globulin antibody (ab107368)
      Immunocytochemistry/ Immunofluorescence - Anti-Cortisol Binding Globulin antibody (ab107368)

      ICC/IF image of ab107368 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107368, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Cortisol Binding Globulin antibody (ab107368)
      Western blot - Cortisol Binding Globulin antibody (ab107368)
      Anti-Cortisol Binding Globulin antibody (ab107368) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

      Predicted band size: 45 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortisol Binding Globulin antibody (ab107368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortisol Binding Globulin antibody (ab107368)
      IHC image of ab107368 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献

    This product has been referenced in:
    • Verhoog N  et al. Inhibition of corticosteroid-binding globulin gene expression by glucocorticoids involves C/EBPß. PLoS One 9:e110702 (2014). Read more (PubMed: 25335188) »

    See 1 Publication for this product

    ab107368 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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