Anti-Corticotropin Releasing Factor Receptor 2 抗体 (ab104368)

製品の概要

  • 製品名
    Anti-Corticotropin Releasing Factor Receptor 2 antibody
    Corticotropin Releasing Factor Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Corticotropin Releasing Factor Receptor 2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Horse, Hamster, Cow, Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Corticotropin Releasing Factor Receptor 2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

画像

  • All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Forebrain
    Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 51 + 54 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    Corticotropin Releasing Factor Receptor 2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.

プロトコール

参考文献

ab104368 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% BIS TRIS)
Loading amount
28 µg
Specification
intestinal tissue
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

投稿 May 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録