Anti-CRHR2 抗体 (ab104368)
Key features and details
- Rabbit polyclonal to CRHR2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
-
製品名
Anti-CRHR2 antibody
CRHR2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CRHR2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Horse, Hamster, Cow, Dog, Human -
免疫原
Synthetic peptide corresponding to Rat CRHR2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125890) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104368の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
-
機能
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 521831 Cow
- Entrez Gene: 482384 Dog
- Entrez Gene: 100055029 Horse
- Entrez Gene: 1395 Human
- Entrez Gene: 12922 Mouse
- Entrez Gene: 64680 Rat
- Omim: 602034 Human
- SwissProt: Q13324 Human
see all -
別名
- corticotropin releasing factor receptor antibody
- Corticotropin releasing hormone receptor 2 antibody
- Corticotropin-releasing factor receptor 2 antibody
see all
画像
-
All lanes : Anti-CRHR2 antibody (ab104368) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain
Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 51,54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsCRHR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab104368 は 4 報の論文で使用されています。
- Lovell AJ et al. The effects of diet and chronic exercise on skeletal muscle ghrelin response. Metabol Open 14:100182 (2022). PubMed: 35340718
- Hucik B et al. Regulation of adipose tissue lipolysis by ghrelin is impaired with high-fat diet feeding and is not restored with exercise. Adipocyte 10:338-349 (2021). PubMed: 34224298
- Cervone DT et al. Unacylated ghrelin stimulates fatty acid oxidation to protect skeletal muscle against palmitate-induced impairment of insulin action in lean but not high-fat fed rats. Metabol Open 5:100026 (2020). PubMed: 32812929
- Castillero E et al. Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery. Am J Physiol Heart Circ Physiol 315:H1463-H1476 (2018). PubMed: 30141986