Anti-Cortactin (phospho Y421) 抗体 (ab47768)
Key features and details
- Rabbit polyclonal to Cortactin (phospho Y421)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Cortactin (phospho Y421) antibody
Cortactin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cortactin (phospho Y421) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Cortactin aa 350-450 (phospho Y421).
Database link: Q14247 -
ポジティブ・コントロール
- Hela cell extract.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47768の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa).
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa). |
ターゲット情報
-
機能
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases. -
配列類似性
Contains 7 cortactin repeats.
Contains 1 SH3 domain. -
ドメイン
The SH3 motif may mediate binding to the cytoskeleton. -
翻訳後修飾
Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia. - Information by UniProt
-
参照データベース
- Entrez Gene: 2017 Human
- Entrez Gene: 13043 Mouse
- Entrez Gene: 60465 Rat
- GenBank: NM_138565 Human
- GenBank: NM_007803 Mouse
- Omim: 164765 Human
- SwissProt: Q14247 Human
- SwissProt: Q60598 Mouse
see all -
別名
- Amplaxin antibody
- CTTN antibody
- EMS 1 antibody
see all
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab47768 は 7 報の論文で使用されています。
- Zhou Z et al. Autophagy promotes invadopodia formation in human ovarian cancer cells via the p62-extracellular signal-regulated kinase 1/2 pathway. Exp Ther Med 22:952 (2021). PubMed: 34335894
- Masi I et al. Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK. Cell Rep 34:108800 (2021). PubMed: 33657382
- Liu Y et al. Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling. Elife 9:N/A (2020). PubMed: 32844749
- Klomp JE et al. Time-Variant SRC Kinase Activation Determines Endothelial Permeability Response. Cell Chem Biol 26:1081-1094.e6 (2019). PubMed: 31130521
- Chellini L et al. Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signalling in ovarian cancer. Matrix Biol 81:17-33 (2019). PubMed: 30367951
- Meiler E et al. Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading. PLoS One 7:e33662 (2012). WB . PubMed: 22479425
- García-Castillo J et al. HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation. J Biol Chem 284:25302-13 (2009). WB ; Human . PubMed: 19589785