The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/30.
ICC: 1/100 - 1/250.
WB: 1/5000. Detects bands of approximately 75 and 80 kDa (predicted molecular weight: 80 kDa).
Is unsuitable for IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Mammary tumor and squamous cell carcinoma associated antibody
Oncogene EMS1 antibody
p80/85 src substrate antibody
Src substrate cortactin antibody
Western blot - Anti-Cortactin antibody [EPR1923Y] (ab68438)
Predicted band size : 80 kDa
Lane 1: Wild-type HAP1 cell lysate (40 µg) Lane 2: Cortactin knockout HAP1 cell lysate (40 µg) Lane 3: NIH3T3 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68438 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68438 was shown to specifically recognize Cortactin in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. Ab68438 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Cortactin antibody [EPR1923Y] (ab68438)
All lanes : Anti-Cortactin antibody [EPR1923Y] (ab68438) at 1/5000 dilution
Lane 1 : C6 cell lysate Lane 2 : MCF-7 cell lysate Lane 3 : Hela cell lysate Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 80 kDa Observed band size : 80 kDa Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.