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RabMAb

Anti-Cortactin 抗体 [EP1922Y] (ab81208)

製品の概要

  • 製品名
    Anti-Cortactin antibody [EP1922Y]
    Cortactin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1922Y] to Cortactin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Cortactin aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: HeLa cell lysate. IHC-P: Human breast carcinoma. ICC/IF: MCF7 and wild-type HAP1 cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/100000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/1000.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/40.

ターゲット情報

  • 機能
    Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • 配列類似性
    Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • ドメイン
    The SH3 motif may mediate binding to the cytoskeleton.
  • 翻訳後修飾
    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

画像

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Cortactin knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH3T3 cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab81208 observed at 62 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab81208 was shown to specifically react with Cortactin in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. ab81208 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • ab81208 staining Cortactin in wild-type HAP1 cells (top panel) and Cortactin knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab81208 at 1/1000 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-Cortactin antibody [EP1922Y] (ab81208) at 1/100000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemical staining of paraffin-embedded human breast carcinoma using 1/100 ab81208.
  • Immunofluorescent staining of MCF7 cells using 1/100 ab81208
  • Overlay histogram showing HeLa cells stained with ab81208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81208, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Chen Q  et al. Growth-induced stress enhances epithelial-mesenchymal transition induced by IL-6 in clear cell renal cell carcinoma via the Akt/GSK-3ß/ß-catenin signaling pathway. Oncogenesis 6:e375 (2017). Read more (PubMed: 28846080) »
  • Sánchez A  et al. Cyclic expression of the voltage-gated potassium channel KV10.1 promotes disassembly of the primary cilium. EMBO Rep 17:708-23 (2016). Read more (PubMed: 27113750) »

See all 9 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
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投稿 May 30 2018

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Human Cell (Ovarian carcinoma (A2780))
Specification
Ovarian carcinoma (A2780)
Permeabilization
Yes - 0.1% Triton-x100
Fixative
Paraformaldehyde
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投稿 Aug 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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