製品の概要

  • 製品名Anti-Cortactin antibody
    Cortactin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cortactin
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 450-500 of Human Cortactin (NP_005222.2).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells and 293T cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab93796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • 配列類似性Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • ドメインThe SH3 motif may mediate binding to the cytoskeleton.
  • 翻訳後修飾Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • 参照データベース
  • 別名
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Anti-Cortactin antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Cortactin with ab93796 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-Cortactin antibody (ab93796) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 62 kDa


    Exposure time : 10 seconds
  • Detection of Cortactin in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93796 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 seconds.

Anti-Cortactin antibody (ab93796) 使用論文

ab93796 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"