製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cortactin
  • アプリケーション
    適用あり: WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to the N-terminal region of human cortactin (amino acids 18-35) conjugated to KLH.

  • ポジティブ・コントロール
    • Human epidermoid carcinoma, A431 cell line for Western blotting, the mouse fibroblasts NIH3T3 cell line for immunofluorescence.
  • 特記事項
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 15mM sodium azide.
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 65-75 kDa. This is determined using a rat brain cytosol extract, a whole extract of chicken embryo fibroblasts, or a whole cell extract of the human epidermoid carcinoma A431 cell line.
ICC/IF Use at an assay dependent dilution. PubMed: 20689769
IP Use at an assay dependent dilution. PubMed: 16971514

ターゲット情報

  • 機能
    Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • 配列類似性
    Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • ドメイン
    The SH3 motif may mediate binding to the cytoskeleton.
  • 翻訳後修飾
    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

参考文献

This product has been referenced in:
  • Ponceau A  et al. aII-spectrin regulates invadosome stability and extracellular matrix degradation. PLoS One 10:e0120781 (2015). Read more (PubMed: 25830635) »
  • Spillane M  et al. Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex. J Neurosci 32:17671-89 (2012). Read more (PubMed: 23223289) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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