製品の概要

  • 製品名
    Anti-Cortactin antibody [4F11]
    Cortactin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4F11] to Cortactin
  • 特異性
    The clone number has been updated from (0.T.21) to (4F11) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: ICC/IF, WB, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Hamster, Cow, Human
  • 免疫原

    Mixture of affinity purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60 src.

  • ポジティブ・コントロール
    • WB: 3T3 and src transformed chicken fibroblast cell lysates. ICC/IF: Wildtype HAP1 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.025% Sodium Azide
    Constituents: 40% Glycerol, 75mM Sodium chloride, 50mM Tris glycine. pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    4F11
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 61 kDa. Use 20 µg per lane of 3T3 cell lysate as a positive control for mini gels.
Note: ab33333 recognizes 80kD and 85kD proteins in avian cells, but it recognizes an 80kD protein in rodent and human cells.
IP Use at 10 µg/mg of lysate.
ICC Use a concentration of 10 µg/ml.

ターゲット情報

Anti-Cortactin antibody [4F11] 画像

  • ab33333 staining Cortactin in wild-type HAP1 cells (top panel) and CTTN knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab33333 at 1μg/ml and ab7291 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-Cortactin antibody [4F11] (ab33333) at 1/1000 dilution + Bovine aortic endothelial whole cell lysates at 10 µg

    Secondary
    Goat anti-mouse polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Additional bands at : 80 kDa (possible post-translational modification).

    Exposure time : 5 seconds

    Image is courtesy of an anonymous AbReview

    Blocking was with 5% milk for 1 hour at 20°C.

    See Abreview

  • ab33333 staining Cortactin (red) in Human palladin-activated fibroblasts by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) before incubation with a Rhodamine Red IgG (1/100).

Anti-Cortactin antibody [4F11] (ab33333) 使用論文

This product has been referenced in:
  • Rosenberg BJ  et al. Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells. Mol Biol Cell 28:1347-1360 (2017). Read more (PubMed: 28356423) »
  • Weidmann MD  et al. Mena(INV) dysregulates cortactin phosphorylation to promote invadopodium maturation. Sci Rep 6:36142 (2016). Read more (PubMed: 27824079) »

See all 15 Publications for this product

Product Wall

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS7 cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
COS7 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 16 2015

Application
Western blot
Sample
Dog Cell lysate - whole cell (Madin-Darby Canine Kidney (MDCK) epithelial cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Madin-Darby Canine Kidney (MDCK) epithelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 29 2015

Application
Western blot
Sample
Cow Cell lysate - whole cell (Bovine aortic endothelial cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Bovine aortic endothelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 25 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 0.5% · Temperature: 20°C
Sample
Mouse Cell (J774 macrophage)
Specification
J774 macrophage
Permeabilization
Yes - 0.05% saponin
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 23 2014

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

Would you be able to provide the lot number and order number?

It may be possible that the ˜100 kDa band corresponds to the pho...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録