製品の概要

  • 製品名Anti-Coronin 1a antibody
    Coronin 1a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Coronin 1a
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 360-462 of Human Coronin 1a

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21169636

ターゲット情報

  • 機能May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
  • 組織特異性Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
  • 配列類似性Belongs to the WD repeat coronin family.
    Contains 7 WD repeats.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actin binding protein antibody
    • CLABP antibody
    • Clipin A antibody
    • Clipin-A antibody
    • CLIPINA antibody
    • COR1A_HUMAN antibody
    • CORO1A antibody
    • Coronin 1 antibody
    • Coronin actin binding protein 1A antibody
    • Coronin like protein A antibody
    • Coronin like protein p57 antibody
    • Coronin-1A antibody
    • Coronin-like protein A antibody
    • Coronin-like protein p57 antibody
    • FLJ41407 antibody
    • HCORO1 antibody
    • MGC117380 antibody
    • OTTHUMP00000163017 antibody
    • p57 antibody
    • TACO antibody
    • Tryptophan aspartate containing coat protein antibody
    • Tryptophan aspartate-containing coat protein antibody
    see all

Anti-Coronin 1a antibody 画像

  • Coronin 1a antibody (ab56820) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.


  • Predicted band size : 51 kDa
  • All lanes : Anti-Coronin 1a antibody (ab56820) at 1/500 dilution

    Lane 1 : Mouse macrophage whole cell lysate
    Lane 2 : Mouse macrophage whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Mouse
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

  • ab56820 staining Coronin 1a in HL-60 cells by Immunocytochemistry/ Immunofluorescence.
    The cells were washed three times with PBS after the phagocytosis assay and fixed with 3.3% paraformaldehyde for 15 minutes. Cells were washed twice with PBS containing 1 mm MgCl2 and 0.1% BSA (staining buffer), permeabilized with 0.2% Triton X-100 in staining buffer for 3 minutes, and blocked with PBS containing 1 mm MgCl2 and 5% BSA for 30 minutes. To assess the distribution of Coronin 1a, a phagocytosis assay was started as described above, and the cells were treated with C3bi-zymosan for 10 minutes at 37 °C. After washing twice to remove unbound C3bi-zymosan particles, the treated cells were reincubated for the indicated times at 37 °C and then fixed and permeabilized. Cells were incubated with ab56820 for 1 hour and then with an Alexa Fluor 488-conjugated phalloidin. For more details please see journal article.

Anti-Coronin 1a antibody (ab56820) 使用論文

This product has been referenced in:
  • Bosedasgupta S & Pieters J Inflammatory stimuli reprogram macrophage phagocytosis to macropinocytosis for the rapid elimination of pathogens. PLoS Pathog 10:e1003879 (2014). Read more (PubMed: 24497827) »
  • Yokoyama K  et al. Rab27a Negatively Regulates Phagocytosis by Prolongation of the Actin-coating Stage around Phagosomes. J Biol Chem 286:5375-82 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21169636) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages)
Loading amount 10 µg
Specification macrophages
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 12 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Formic acid followed by autoclave in citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Sílvia Sisó

Verified customer

投稿 Feb 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"