1/5000. Using ABC or other enzyme linked immunocytochemical procedures.
1/500 - 1/1000.
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
Belongs to the WD repeat coronin family. Contains 7 WD repeats.
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
ab123574, at 1/10000, staining Coronin 1a (green) in a mixed neuron/glial culture from newborn rat brain, by immunocytochemistry. Red staining is from an anti-Vimentin antibody, while blue is a nuclear DNA counter stain.
Western blot - Anti-Coronin 1a antibody (ab123574)