Rabbit polyclonal to CORO1B
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Corresponds to a region between amino acids 450-489 of Human CORO1B (NP_065174.1).
HeLa, 293T and NIH 3T3 whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
Use at 2-5 µg/mg of lysate.
Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
Belongs to the WD repeat coronin family.
Contains 5 WD repeats.
Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
Information by UniProt
CORO 1B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CORO1B antibody (ab99407)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of humancolon carcinoma tissue labelling CORO1B with ab99407 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - Anti-CORO1B antibody (ab99407)
All lanes : Anti-CORO1B antibody (ab99407) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 54 kDa Exposure time: 3 seconds
Immunoprecipitation - Anti-CORO1B antibody (ab99407)
ab99407 at 1µg/ml detecting CORO1B in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CORO1B
Lane 2: ab99407 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 1 second.
has not yet been referenced specifically in any publications.
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