製品の概要
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製品名Anti-CORO1B antibody
CORO1B 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CORO1B
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アプリケーション適用あり: IHC-P, WB, IPmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Corresponds to a region between amino acids 450-489 of Human CORO1B (NP_065174.1).
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ポジティブ･コントロール
- HeLa, 293T and NIH 3T3 whole cell lysates
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab99407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 54 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
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配列類似性Belongs to the WD repeat coronin family.
Contains 5 WD repeats. -
翻訳後修飾Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
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細胞内局在Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
- Information by UniProt
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参照データベース
- Entrez Gene: 57175 Human
- Entrez Gene: 23789 Mouse
- Omim: 609849 Human
- SwissProt: Q9BR76 Human
- SwissProt: Q9WUM3 Mouse
- Unigene: 6191 Human
- Unigene: 276859 Mouse
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別名
- COR1B_HUMAN antibody
- CORO 1B antibody
- CORO1B antibody
see all
Anti-CORO1B antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CORO1B antibody (ab99407)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of humancolon carcinoma tissue labelling CORO1B with ab99407 at 1/1000 (0.2µg/ml). Detection: DAB. -
Western blot - CORO1B antibody (ab99407)All lanes : Anti-CORO1B antibody (ab99407) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 54 kDa
Exposure time : 3 seconds -
Immunoprecipitation - CORO1B antibody (ab99407)ab99407 at 1µg/ml detecting CORO1B in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CORO1B
Lane 2: ab99407 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 1 second.
Anti-CORO1B antibody (ab99407) 使用論文
ab99407 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"