Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of humancolon carcinoma tissue labelling CORO1B with ab99407 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - CORO1B antibody (ab99407)
All lanes : Anti-CORO1B antibody (ab99407) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 seconds
Immunoprecipitation - CORO1B antibody (ab99407)
ab99407 at 1µg/ml detecting CORO1B in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CORO1B
Lane 2: ab99407 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 1 second.