Anti-CORO1B 抗体 (ab119714)
Key features and details
- Rabbit polyclonal to CORO1B
- Suitable for: WB, ICC/IF
- Reacts with: Rabbit, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CORO1B antibody -
製品の詳細
Rabbit polyclonal to CORO1B -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rabbit, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide, coupled to carrier protein, corresponding to amino acids at the C terminus of Human CORO1B.
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ポジティブ・コントロール
- Human A431 and HeLa cell lysates; Mouse brain extracts and Rabbit spleen fibroblasts.
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特記事項
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab119714 was affinity purified using CORO1B (C-terminus) peptide (without carrier). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119714の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 54 kDa.
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|
ICC/IF |
1/100.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100. |
ターゲット情報
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機能
Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction. -
配列類似性
Belongs to the WD repeat coronin family.
Contains 5 WD repeats. -
翻訳後修飾
Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. -
細胞内局在
Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 57175 Human
- Entrez Gene: 100009415 Rabbit
- Entrez Gene: 29474 Rat
- Omim: 609849 Human
- SwissProt: Q9BR76 Human
- SwissProt: O89046 Rat
- Unigene: 6191 Human
- Unigene: 2819 Rat
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別名
- COR1B_HUMAN antibody
- CORO 1B antibody
- CORO1B antibody
see all
画像
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Lanes 1-2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilution
Lanes 3-4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution
Lanes 1 & 3 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min
Lanes 2 & 4 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min before
treatment with lambda phosphatase
Predicted band size: 54 kDa -
ab119714 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to Cy3.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab119714 は 1 報の論文で使用されています。
- Striepen JF & Voeltz GK Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission. J Cell Biol 221:N/A (2022). PubMed: 35802042