製品の概要

  • 製品名Anti-CoREST3 / RCOR3 antibody
    CoREST3 / RCOR3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CoREST3 / RCOR3
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 445 and 495 of human CoREST3/ RCOR3 (NP_060724.1), (and between residues 503-553 NP_001129695.1.).

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500.
ICC 1/50 - 1/250.

Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

ICC/IF 1/50 - 1/250.

ターゲット情報

  • 関連性The CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
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Anti-CoREST3 / RCOR3 antibody 画像

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
  • All lanes : Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second
  • Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.
    ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-CoREST3 / RCOR3 antibody (ab76921) 使用論文

This product has been referenced in:
  • Sáez JE  et al. Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation. PLoS One 10:e0131760 (2015). Read more (PubMed: 26111147) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"