Anti-CoREST3 / RCOR3 抗体 (ab50197)

製品の概要

  • 製品名Anti-CoREST3 / RCOR3 antibody
    CoREST3 / RCOR3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CoREST3 / RCOR3
  • 特異性This antibody reacts with CoREST3/RCOR3
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MRVGAEYQAR IPEFDPGATK YTDKDNGGML VWSPYHSIPD AKLDEYIAIA, corresponding to N terminal amino acids 1-50 of Human CoREST3/ RCOR3

  • ポジティブ・コントロール
    • Jurkat cell lysate and human spleen tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/62500.

ターゲット情報

  • 関連性The CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • 4921514E24Rik antibody
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    • E130101E15Rik antibody
    • FLJ10876 antibody
    • FLJ16298 antibody
    • KIAA1343 antibody
    • MGC28186 antibody
    • OTTHUMP00000035053 antibody
    • RCOR3 antibody
    • REST corepressor 3 antibody
    • RP11-318L16.1 antibody
    see all

Anti-CoREST3 / RCOR3 antibody 画像

  • Anti-CoREST3 / RCOR3 antibody (ab50197) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CoREST3 / RCOR3 with ab50197 at 4-8µg/ml. Arrows indicate positive staining.

Anti-CoREST3 / RCOR3 antibody (ab50197) 使用論文

ab50197 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Whole Brain Homogenate)
Gel Running Conditions Reduced Denaturing (8% gel)
Loading amount 10 µg
Specification Whole Brain Homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Martin Engel

Verified customer

投稿 Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"